Mechanism research of soybean seedling cold acclimation through pretreatment of soybean seeds with He-Ne laser

Guo Jinhua; Yin Ruochun; Xu Jian; Yu Zengliang

LASER TECHNOLOGY > 2003 > 27(6): 506-509.

Guo Jinhua, Yin Ruochun, Xu Jian, Yu Zengliang. Mechanism research of soybean seedling cold acclimation through pretreatment of soybean seeds with He-Ne laser[J]. LASER TECHNOLOGY, 2003, 27(6): 506-509.

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Mechanism research of soybean seedling cold acclimation through pretreatment of soybean seeds with He-Ne laser

1.
Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, CAS, Hefei, 230031;
2.
Department of Biotechnology, School of Life Science, Anhui University, Hefei, 230039;
3.
Anhui Fengle Agricultural Science and Technology Academy, Hefei, 230031

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Received Date: March 20, 2003
Revised Date: April 29, 2003
Published Date: November 24, 2003

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Abstract

Abstract

How laser pretreatment can improve soybean seedlings' cold acclimation is studied in the view of energy transformation,biomembrane integrality and oxygen free radical injury.The results show that pretreatment of soybean seeds with He-Ne laser enhance not only the seeds' content of ATP but also seedlings' cold acclimation during cotyledons to primary leaves stage.Pretreatment groups' seedlings at 3℃～5℃ temperature have much higher enzyme activities and content of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD),amylase(AML) and soluble protein content than those in control.Pretreatment of soybean seeds with laser also decreases the rate of product of O2-,the accumulation of MDA and the conductivity rate at low temperature.
- laser biology,
- mechanism of cold acclimation,
- pretreatment seed with laser,
- ATP,
- free radical,
- enzymes activity,
- membrane permeability,
- soybean seedling

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