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光敏化BPD-MA后膀胱癌细胞凋亡的研究
Apoptosis of human bladder cancer BIU-87 cells after photodynamic therapy with laser-activated BPD-MA determined with TUNEL assays
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摘要: 为了研究光动力抑癌作用机制,采用流式细胞仪和TUNEL法检测激光光敏化BPD-MA后人膀胱癌细胞株BIU-87细胞凋亡的发生状况.激光光敏化BPD-MA后人膀胱癌细胞株BIU-87组细胞凋亡发生率明显增高,许多细胞核呈棕黄色,高倍镜下可见细胞核内有大量棕褐色颗粒,与阳性对照中凋亡细胞核的特征相符合,而对照组此种细胞少见.TUNEL阴性对照中细胞核均呈蓝色.结果表明,激光激活BPD-MA光动力作用明显诱导人膀胱癌细胞株BIU-87细胞发生凋亡,这可能是其抑癌作用机制之一.Abstract: In order to study the mechanism of photodynamic therapy on malignant tumors,photosensitization of BPD-MA is activated by laser with red light(632.8nm) delivered at 10mW/cm2to give a total dose of 2.4J/cm2.Apoptosis of human bladder cancer BIU-87 cells is determined with flow cytometry with PI staining and terminal deoxyuridine nick end labeling(TUNEL) assays.Results show that photodynamic therapy with BPD-MA significantly increases the rate of apoptosis in BIU-87 cells.A lot of the positive cells in which their nucleus is brown-yellow fine particle shape or overflowing-dispersed are observed after treatment of cells with photodynamic therapy with BPD-MA.However,laser irradiation alone,BPD-MA alone and sham radiation without BPD-MA can not affect apoptosis of human bladder cancer BIU-87 cells.A conclusion can be made that treatment of cells with photodynamic therapy obviously induces human bladder cancer BIU-87 cells to apoptosis,which may be one of the important mechanism of photodynamic therapy on malignant tumors.
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Key words:
- medical optics and biotechnology /
- BPD-MA /
- photodynamic therapy /
- bladder cancer /
- apoptosis
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