偏振荧光光谱在肝病诊断中的应用研究

基金项目:

国家自然科学基金资助项目(60278037)

详细信息

Study on the polarization fluorescence spectra used for the liver disease diagnosis

1.
Department of Physics, Dalian University of Technology, Dalian 116024, China;
2.
the 201th Hospital of PLA, Dalian 116021, China

摘要: 利用488.0nm激发光诱导肝病变患者及健康人血清的自体荧光,在495nm~640nm范围内进行荧光光谱、水平和垂直偏振荧光光谱采集.实验结果表明,肝病变患者血清的荧光光谱带宽与正常人的相比差异不明显.但是,肝病患者血清的水平和垂直偏振荧光光谱的半峰全宽和0.707带宽(强度为1/2时的带宽)都比健康人血清的带宽宽.这一结果对于肝病的诊断研究有一定的参考价值.
- 偏振荧光光谱 /
- 自体荧光 /
- 半峰全宽 /
- 带宽 /
- 血清
Abstract: The human serum polarization fluorescence spectra(SPFS) are excited with 488.0nm Ar-ion laser and collected between 495nm and 640nm both in horizontal and vertical polarization direction respectively.The bandwidth of the SPFS are calculated both in the full width at half maximum and 0.707 bandwidth in intensity.The SPFS from the abnormal are always wider than those from the healthy.However,the difference in the serum fluorescence spectra(SFS) is not obvious.This above result is a valuable reference in the future liver hypohepatia diagnosis.
- polarization fluorescence spectra /
- autofluorescence /
- FWHM /
- bandwidth /
- serum

[1]	程明亮,陆彤,梁跃东 et al.肝纤维化发生机制的研究进展[J].科技导报,1999(5):22～24.
[2]	焦锡莹,高泽红,丁建华 et al.应用激光诱发荧光诊断不同物质导致的白鼠肝纤维化[J].激光杂志,2001,22(4):47～48.
[3]	冯兆池,丁建华,余虹 et al.恶性肿瘤患者血清特异可见荧光光谱的初步研究[J].大连理工大学学报,1997,37(4):403～405.
[4]	赵箭光,吴晓玲,杨佩青 et al.肝癌与正常肝组织激光荧光光谱的比较研究[J].中国医学物理学杂志,1996,13(4):220～222.
[5]	赵晓杰,李少白,江山 et al.激光诱导血清荧光诊断肝癌初探[J].光电子·激光,1994,5(1):31～33.
[6]	新谷隆一.偏振光[M].北京:原子能出版社,1994.6～7.
[7]	杜鸣,唐波,沈含熙.偏振荧光光度法同时测定氨基酸注射液及动、植物浸出液中酪氨酸、色氨酸的研究[J].药学学报,1997,32(9):695～698.
[8]	PRADHAN A,PANDA R N,NAIR M S et al.Fluorescence study of normal,benign and malignant human breast tissues[J].Proc SPIE,2000,3917:240～243.
[9]	刘桪,陈丽娜,李冰 et al.慢性肝病患者血卟啉变化初探[J].哈尔滨医科大学学报,1992,26(2):99～101.

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