偏振荧光光谱在肝病诊断中的应用研究

Study on the polarization fluorescence spectra used for the liver disease diagnosis

摘要: 利用488.0nm激发光诱导肝病变患者及健康人血清的自体荧光,在495nm~640nm范围内进行荧光光谱、水平和垂直偏振荧光光谱采集.实验结果表明,肝病变患者血清的荧光光谱带宽与正常人的相比差异不明显.但是,肝病患者血清的水平和垂直偏振荧光光谱的半峰全宽和0.707带宽(强度为1/2时的带宽)都比健康人血清的带宽宽.这一结果对于肝病的诊断研究有一定的参考价值.

Abstract: The human serum polarization fluorescence spectra(SPFS) are excited with 488.0nm Ar-ion laser and collected between 495nm and 640nm both in horizontal and vertical polarization direction respectively.The bandwidth of the SPFS are calculated both in the full width at half maximum and 0.707 bandwidth in intensity.The SPFS from the abnormal are always wider than those from the healthy.However,the difference in the serum fluorescence spectra(SFS) is not obvious.This above result is a valuable reference in the future liver hypohepatia diagnosis.