Advanced Search

ISSN1001-3806 CN51-1125/TN Map

Volume 31 Issue 1
May  2010
Article Contents
Turn off MathJax

Citation:

Mutagenesis and selection of chondroitinase producing strain of Serratia Marcescens by CO2 laser irradiation

  • Received Date: 2005-11-08
    Accepted Date: 2006-03-06
  • Serratia Marcescens GT596 was isolated from soil and brought to mutation by CO2 laser irradiation.The radiation duration was 30s with distance of 23cm and the flash beam diameter was 0.4cm.High chondrotinase producing strain mutant GT596 was obtained after screening culture.The GT596 was found to have chondrotinase stable producing activity in descendant cultures.
  • 加载中
  • [1]

    TAN X S,WU Q H,LIANG Y J et al.Research on the etiology of Kaschin-Beek disease[J].Chinese Journal of Microecology,1994,6(2):44~45(in Chinese).
    [2]

    QI L C,LIN X S,LIC S.Study on composition and properties of glucosa mineglycan in lung cancer[J].Journal of Bethune Univisity Medical Science,1999,25 (1):44~46.
    [3]

    KATO F,IWATE H,MIMASTSLL K et al.Chemonulealysis with chondroitinase ABC:comparative study with chymopapain[J].The Journal of Orthopaedis Surgery and Ttaumatology,1988 (31):63.
    [4]

    YAMAGATA T,SAITO H,HABUCHI O et al.Purification and properties of bacterial chondroitinases and chondrosulfatases[J].The Journal of Biological Chemistry,1968,243 (7):1523~1535.
    [5]

    ZONG R H,Infrared laser and organism mutual action mechanism discussion[J].Laser Magazine,1988,9 (6):47~48 (in Chinese).
    [6]

    WANG R L,OU J M,SHANG H L et al.Laser illumination in fine root thunder strain selective breeding applied research[J].Laser Magazine,1998,19 (4):44~46 (in Chinese).
    [7]

    TAO K,WANG Zh Y,GUO J L et al.Study on the screening of chondroitinase ABC producing strain and their fermentation technology[J].Chinese Journal of Antibiotic,2004,29 (3):138~141 (in Chinese).
    [8]

    HU W H,CHEN Y,YANG Z Sh et al.CO2 laser illumination yeast biology effect[J].Laser Magazine,2001,21 (3):31~33 (in Chinese).
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(3650) PDF downloads(555) Cited by()

Proportional views

Mutagenesis and selection of chondroitinase producing strain of Serratia Marcescens by CO2 laser irradiation

  • 1. College of Life Science, Sichuan University, Chengdu 610064, China;
  • 2. Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China

Abstract: Serratia Marcescens GT596 was isolated from soil and brought to mutation by CO2 laser irradiation.The radiation duration was 30s with distance of 23cm and the flash beam diameter was 0.4cm.High chondrotinase producing strain mutant GT596 was obtained after screening culture.The GT596 was found to have chondrotinase stable producing activity in descendant cultures.

Reference (8)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return