Mutagenesis and selection of chondroitinase producing strain of Serratia Marcescens by CO2 laser irradiation

Serratia Marcescens GT596 was isolated from soil and brought to mutation by CO2 laser irradiation.The radiation duration was 30s with distance of 23cm and the flash beam diameter was 0.4cm.High chondrotinase producing strain mutant GT596 was obtained after screening culture.The GT596 was found to have chondrotinase stable producing activity in descendant cultures.