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Volume 31 Issue 5
May  2010
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Citation:
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Computer processing and visualization of confocal microscopy images

  • 1.

    College of Automation Engineering, Nanjing University of Aeronautics & Astronautics, Nanjing 210016, China;

  • 2.

    College of Electrical Engineering, Nantong University, Nantong 226007, China

  • Corresponding author: WANG Hui-nan, wanghn_nuaa@sina.com
  • Received Date: 2006-08-16
    Accepted Date: 2006-11-06
  • The methodology was proposed for image processing and interactive visualization of confocal microscopy images.Firstly,an intensity compensation algorithm was applied to reduce noises resulted from light absorption and scattering by objects and particles in the volume through which light passes.Secondly,a deconvolution algorithm based on maximum a posteriori was applied to improve the image stacks quality.At last,the ray-casting algorithm was used to reconstruct the fine details in the volume.Furthermore,virtual reality technique was integrated with volume rendering in order to provide users an intuitive interface to view the cells and its structures through translation,rotation and zoom operations.The speed and versatility of the program allows for convenient,fast,interactive examination of unknown cell structures even on common PC.The program can be applied for different types of confocal microscopy data sets with good results.
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  • [1]

    SAKAS G,VICKER M G,PLATH P J.Case study:visualization of laser confocal microscopy datasets[A].Proceedings of the 7th IEEE Visualization Conference[C].San Francisco:IEEE Press,1996.375～379.
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    SUN Y L,RAJWA B,ROBINSON J P.Adaptive image-processingtechnique and effective visualization of confocal microscopy images[J].Microscopy Research and Technique,2004,64(2):156～163.
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    RAZDAN A,PATEL K,FARIN G E et al.Volume visualization of multicolor laser confocal microscope data[J].Computers & Graphics,2001,25(3):371～382.
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    FANG S F,DAI Y,MYERS F et al.Three-dimensional microscopy data exploration by interactive volume visualization[J].Scanning,2000,22(4):218～226.
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    RICHARD O D,PETER E H,DAVID G S.Pattern classification[M].2nd ed,New York:John Wiley & Sons Inc,2001.124～128.
    [6]

    YU D Y.Image processing and study on microscopy images[D].Nanjing:Nanjing University of Science and Technology,2002.23～35(in Chinese).
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Computer processing and visualization of confocal microscopy images

    Corresponding author: WANG Hui-nan, wanghn_nuaa@sina.com
  • 1. College of Automation Engineering, Nanjing University of Aeronautics & Astronautics, Nanjing 210016, China;
  • 2. College of Electrical Engineering, Nantong University, Nantong 226007, China
  • Received Date: 2006-08-16
  • Accepted Date: 2006-11-06
  • Available Online: 2007-10-25

Abstract: The methodology was proposed for image processing and interactive visualization of confocal microscopy images.Firstly,an intensity compensation algorithm was applied to reduce noises resulted from light absorption and scattering by objects and particles in the volume through which light passes.Secondly,a deconvolution algorithm based on maximum a posteriori was applied to improve the image stacks quality.At last,the ray-casting algorithm was used to reconstruct the fine details in the volume.Furthermore,virtual reality technique was integrated with volume rendering in order to provide users an intuitive interface to view the cells and its structures through translation,rotation and zoom operations.The speed and versatility of the program allows for convenient,fast,interactive examination of unknown cell structures even on common PC.The program can be applied for different types of confocal microscopy data sets with good results.

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