Study of carotenoid in human skin by means of resonance Raman detection

Carotenoid molecules are powerful antioxidants that play important role throughout the human body.The level of carotenoid in human body can be used to reflect the human's health condition.Experiments were carried to detect the carotenoid in human.After tests repeatedly,suitable experimental parameters were determined.That is to say the best location to perform the measurement is thrum,laser's wavelength is 473nm,intensity of laser is 20mW and exposure time is 5s.At last obvious resonance Raman spectra of carotenoid in human skin was obtained after the fluorescence background was fitted and subtracted from the original spectra by means of fitted differential algorithm.Experimental results prove that resonance Raman spectra is applicable for carotenoid detection in human.