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Yang Chu-ping. The optical transfer function of a fluorescent confocal microscope with extended Gaussian source[J]. LASER TECHNOLOGY, 2005, 29(5): 552-554.
Citation: Yang Chu-ping. The optical transfer function of a fluorescent confocal microscope with extended Gaussian source[J]. LASER TECHNOLOGY, 2005, 29(5): 552-554.

The optical transfer function of a fluorescent confocal microscope with extended Gaussian source

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  • Received Date: August 08, 2004
  • Revised Date: December 14, 2004
  • Published Date: September 24, 2005
  • The influence of the three parameters,namely the beam size of Gaussian source,the source size and the detector size,on the three-dimensional optical transfer function(3-D OTF) of an incident light fluorescent confocal microscope is investigated,and the 3-D OTF of such a set-up is derived.The calculated results show that the beam size has an effect on choosing both the source size and the detector size by means of a gaussian beam with the smaller beam size,both the source size and the detector size can be increased,and better optical resolution and higher signal-noise-ratio can be reached,compared with those by means of a parallel beam.
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