Analization of blood cells fluorescence spectra induced by LED at the wavelength of 407nm

The fluorescence spectra for whole blood,erythrocyte and hemoglobin from healthy and fresh experimental mice under the wavelength of 407nm (Δλ1/2≈18nm) excitation are measured by a grating spectrometer,and their emitting mechanism and spectral characteristic are investigated. The experimental results and theoretic analization about it are presented in this paper. It shows that the blood or erythrocyte emitting fluorescence quantum yield efficiency under 407nm-LED excitation may reach above 90%,the blood fluorescence mainly result from fluorophores of the erythrocyte,but fluorescence spectra of hemoglobin by 407nm-LED excitation are obviously different from that of erythrocyte,and the fluorescence quantum yield of cracked erythrocyte remarkably declines and its spectral profiles are being distorted with the consistency of the cracked erythrocyte increasing.