A laser micro-beam system for cell manipulation

A laser miro-beam system for cell manipulation is introduced in the paper.It consists of an optical scalpel,which is a Nd:YAG laser beam focused tightly by an objective and an optical trap,the focused He-Ne laser beam. Not only the overall design of the laser micro-beam system but also the design and the choice of the critical components of the system are reviewed.The optical trap can manipulate cells in suspension, it validates the kinetic performance of the laser trap. The optical scalpel can concise chromosome and punch holes in cells or organelles.