Determination of optical coefficients of biological tissue by means of diffuse image

In order to acquire the optical properties (scattering and absorption coefficients) of biological tissue, the CCD non-invasive measurement equipment was used to measure the image of diffuse reflectance of biologrcal tissue. The spatially resolved diffuse reflectance of the tissue was acquired by analyzing the image. One set of experimental data on tissue phantoms were used to train a neural network which was used to extract the optical properties. The accuracy of the neural network was investigated and validated by another set of experimental data. The optical properties of bovine muscle, pig muscle and human skin tissue under 633nm laser irradiation were also determined by the network which was trained by the experiment. The result shows the neural network can estimate the optical properties of different tissues with high accuracy.