Manufacturing method of a phase plate and its application in phase-contrast experiments

For the purpose of producing a phase plate for phase-contrast experiments, firstly, controlling the exposure time and washing process of a holographic plate, the thickness or refractive index of the film was changed so as to adjust the phase change. Secondly, with the help of two-point light sources interference phase measurement, the required phase plate was.detected and screened and its two-dimension phase distribution was obtained. Finally, a π/2 phase plate with a certain absorptivity was selected as a phase filter located in the spectrum surface of the lens in the experiment, and the predicated image was obtained on the image plane. The results in the phase detection and the phase-contrast experiments show that it is feasible to produce a phase plate with a holographic plate,and the phase and absorptivity of the phase plate can be designed at will. This is a simple,cheap and fast process to design, manufacture and measure a phase plate.