Study on measuring surface-charges of cells in a quartz microchannel

CHEN De-liang; FENG Cai-ling; QIN Shui-jie

doi:10.3969/j.issn.1001-3806.2009.06.001

The surface-charges of cells are very important to study the vital characteristic of cells.Common methods only measure the changes of cell electrophoresis mobility (EPM) instead of the quantity of cell surface-charges.In order to measure the surface-charges of cells,a novel method to measure the surface-charges of cells by means of optical tweezers and electrophoresis in a microchannel was designed.With this method,the quantity of the electric charge of yeast cells was measured about 10-10℃.Experimental results prove that this method can be used to measure the changes of cell surface-charges.A lab-onchip (LOC) for measuring the surface-charge of cells might be developed after improving this measuring system.

Study on measuring surface-charges of cells in a quartz microchannel

Corresponding author: QIN Shui-jie, sjqin@mail.gzst.gov.cn

1. Laboratory for Photoelectric Technology and Application of Guizhou, Guizhou University, Guiyang 550025, China

Received Date: 2009-01-06

Accepted Date: 2009-03-01

Available Online: 2009-12-25

Abstract: The surface-charges of cells are very important to study the vital characteristic of cells.Common methods only measure the changes of cell electrophoresis mobility (EPM) instead of the quantity of cell surface-charges.In order to measure the surface-charges of cells,a novel method to measure the surface-charges of cells by means of optical tweezers and electrophoresis in a microchannel was designed.With this method,the quantity of the electric charge of yeast cells was measured about 10-10℃.Experimental results prove that this method can be used to measure the changes of cell surface-charges.A lab-onchip (LOC) for measuring the surface-charge of cells might be developed after improving this measuring system.

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Reference (16)

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Study on measuring surface-charges of cells in a quartz microchannel

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