Study on measuring surface-charges of cells in a quartz microchannel

CHEN De-liang; FENG Cai-ling; QIN Shui-jie

doi:10.3969/j.issn.1001-3806.2009.06.001

CHEN De-liang, FENG Cai-ling, QIN Shui-jie. Study on measuring surface-charges of cells in a quartz microchannel[J]. LASER TECHNOLOGY, 2009, 33(6): 561-564. DOI: 10.3969/j.issn.1001-3806.2009.06.001

Citation:

CHEN De-liang, FENG Cai-ling, QIN Shui-jie. Study on measuring surface-charges of cells in a quartz microchannel[J]. LASER TECHNOLOGY, 2009, 33(6): 561-564. DOI: 10.3969/j.issn.1001-3806.2009.06.001

Citation:

CHEN De-liang, FENG Cai-ling, QIN Shui-jie. Study on measuring surface-charges of cells in a quartz microchannel[J]. LASER TECHNOLOGY, 2009, 33(6): 561-564. DOI: 10.3969/j.issn.1001-3806.2009.06.001

Study on measuring surface-charges of cells in a quartz microchannel

Graphical Abstract

Graphical Abstract

Abstract

Abstract

The surface-charges of cells are very important to study the vital characteristic of cells.Common methods only measure the changes of cell electrophoresis mobility (EPM) instead of the quantity of cell surface-charges.In order to measure the surface-charges of cells,a novel method to measure the surface-charges of cells by means of optical tweezers and electrophoresis in a microchannel was designed.With this method,the quantity of the electric charge of yeast cells was measured about 10-10℃.Experimental results prove that this method can be used to measure the changes of cell surface-charges.A lab-onchip (LOC) for measuring the surface-charge of cells might be developed after improving this measuring system.