Characteristics and composition analysis of pure liquid milk based on 3-D fluorescent spectra
-
摘要: 为了能够快速检测牛奶质量问题,采用3维荧光光谱和多峰拟合成分的方法,对纯牛奶的3维荧光光谱的特征及其主要成分进行了分析研究。结果表明,激发光谱在250nm～310nm范围,最佳激发波长在290nm附近;发射光谱主要在285nm～500nm,具有较强的荧光,荧光峰在330nm附近。液态纯牛奶的荧光光谱主要由5个谱峰（Ⅰ～Ⅴ）组成,其中峰Ⅰ是乳糖影响下部分蛋白质的荧光、峰Ⅱ为脂肪和VE共同产生的、峰Ⅲ为蛋白质直接所为、峰Ⅳ是其它成分影响下的蛋白质的荧光、峰V是VA,VB1和VB6等微量元素共同产生的。研究结果可为液态纯牛奶的荧光光谱检测技术研究提供实验和理论依据。Abstract: To detect the quality of pure milk rapidly, its spectral characteristics were analyzed combining 3-D fluorescent spectra with multi-peak fitting method, and its main compositions were obtained. The results show that its excitation spectrum ranges from 250nm to 310nm, and the peak wavelength is 290nm. The main emission spectrum ranges from 280nm to 500nm with the peak adjacent to 330nm.Meanwhile, the fluorescent spectrum has five spectral peaks(Ⅰ~Ⅴ), where peakⅠis generated by some proteins under the influence of lactose, peak Ⅱis generated by fat and vitamin E jointly, peak Ⅲ is generated by protein directly, peak Ⅳ is generated by some proteins under some unknown influence, and peak Ⅴ is generated jointly by microelements including vitamin A, vitamin B1, and B6. The study results provide some basic principles for studying milk composition with fluorescent spectroscopy.
-
Keywords:
- spectroscopy /
- 3-D fluorescent spectra /
- multi-peak fitting /
- pure liquid milk /
- composition
-
-
[1] HE Y C.Development of Chinese dairy industry[M].Beijing:Science Press,2010:63-78(in Chinese).
[2] ZHANG X L,HUA R,YANG Q L,et al.Hand-held extinction photometer for the detection of idntegrate quality of milk on-site rapidly[J].Analytical Chemistry,2009,37(s1):45(in Chinese).
[3] LLU J,YU C Q,LI J Z,et al.Study on the deteriorating course of fresh milk by laser-induced fluorescence spectra[J].Spectroscopy and Spectral Analysis,2001,21(6):769-771(in Chinese).
[4] CHEN X,GU R,CHEN X,et al.Research on feature extractions of 3-D spectrum of milk[J].China Dairy Industry,2009,37(7):13-15(in Chinese).
[5] LU J,GAO Sh M,XIONG J,et al.Characteristics and mechanism for fluorescence spectroscopy of female urine[J].Laser Technology,2010,34(1):45-47(in Chinese).
[6] LI Y L,DENG L Q,GUO X M,et al.Determination of vitamin E in vegetable by synchronous fluorescence spectrometry[J].Hebei University (Natural Science Edition),2009,29(4):412-415 (in Chinese).
[7] ZHAO W Y,YANG C F,LIU Y.A study on spectral characteristics of saturated fatty acids-ethanol solution[J].Xuzhou Normal University (Natural Science Edition),2009,27(2):55-58(in Chinese).
[8] ZHAO N M,ZHOU H M.Biophysics[M].Beijing:Higher Education Press,2000:300-302(in Chinese).
[9] GU L L,WANG K,WANG M,et al.Fluorescence study on the interaction of galactose and β-galactosides with a galectin from the edible mushroom agrocybe aegerita[J].Analytical Science,2006,22(6):621-625(in Chinese).
[10] WU G H,HE C Y.Application of artificial neural network to simultaneous spectrofluorimetric determination of vitamin B1,B2 and B6[J].Spectroscopy and Spectral Analysis,2003,23(3):535-538(in Chinese).
[11] ZAIDI F,ROUISSI H,DRIDI S,et al.Front-face fluorescence specctroscopy as a rapid and non-destructive tool for differentiating between Sicilo-Sarde and Comisana ewe's milk during lactation period:a preliminary study[J].Food & Bioprocess Technology,2008,1(2):143-151.
计量
- 文章访问数: 1
- HTML全文浏览量: 0
- PDF下载量: 24
下载: