沙雷氏菌产硫酸软骨素酶菌种的CO2激光辐照选育

Mutagenesis and selection of chondroitinase producing strain of Serratia Marcescens by CO2 laser irradiation

摘要: 为了选育高产硫酸软骨素酶菌株,从土壤中首次筛选到一株产硫酸软骨素酶的粘质沙雷氏菌,经过CO2激光辐射诱变(激光功率10W,扩束光斑直径0.5cm,照射距离23cm,照射时间30s),选育出高产硫酸软骨素酶的菌株GT596。经传代培养,发现具有稳定的高产硫酸软骨素酶活性。

Abstract: Serratia Marcescens GT596 was isolated from soil and brought to mutation by CO2 laser irradiation.The radiation duration was 30s with distance of 23cm and the flash beam diameter was 0.4cm.High chondrotinase producing strain mutant GT596 was obtained after screening culture.The GT596 was found to have chondrotinase stable producing activity in descendant cultures.