A model for light scattering from red blood cells
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摘要: 针对球形细胞模型理论与双凹圆盘状的红细胞在实际光散射法检测中的差异,根据细胞的类别特征,提出以扁椭球模型来模拟红细胞,以Rayleigh-Debye-Gans近似理论为基础,引入扁椭球的体极化率,得到了扁椭球模型的散射光公式,并进行数值模拟,研究其光散射特性。结果表明,扁椭球模型更精确地反映了红细胞光散射的真实情况,同时其前向、侧向及后向光散射精细谱的呈现,可以较容易地辨别病理状态的红细胞,能够较好地提高医学检验及诊断的精度,并可以应用于对红细胞的变形性研究。Abstract: Considering the difference between the real blood cells and their spheroid model for the scattering detection of cells,a model was presented to calculate the light scattering properties of nucleated cells which were mimicked by flat ellipsoid.The light-scattering characteristics were derived from the Rayleigh-Debye-Gans approximation and the volume polarizability.Results show that the model is superior to ball model.The model can reflect the true configuration of the blood cells more accurately.In addition,the simultaneous detection of the light-scatter intensities in the forward-scatter,lateral-scatter,and back-scatter directions can help us to distinguish the different cell types from heterogeneous population of blood cells.It can be well used in cell classification and research of cell transfiguration.
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Keywords:
- scattering red blood cell /
- flat ellipsoid /
- body factor
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