高级检索

激光扫描共聚焦显微镜图像的计算机处理

汤敏, 王惠南

汤敏, 王惠南. 激光扫描共聚焦显微镜图像的计算机处理[J]. 激光技术, 2007, 31(5): 558-560.
引用本文: 汤敏, 王惠南. 激光扫描共聚焦显微镜图像的计算机处理[J]. 激光技术, 2007, 31(5): 558-560.
TANG Min, WANG Hui-nan. Computer processing and visualization of confocal microscopy images[J]. LASER TECHNOLOGY, 2007, 31(5): 558-560.
Citation: TANG Min, WANG Hui-nan. Computer processing and visualization of confocal microscopy images[J]. LASER TECHNOLOGY, 2007, 31(5): 558-560.

激光扫描共聚焦显微镜图像的计算机处理

详细信息
    作者简介:

    汤敏(1977- ),女,助教,博士研究生,主要研究三维医学图像可视化及成像.

    通讯作者:

    王惠南,E-mail:wanghn_nuaa@sina.com

  • 中图分类号: TP391

Computer processing and visualization of confocal microscopy images

  • 摘要: 为了研究激光扫描共聚焦显微镜图像的计算机处理,首先对图像进行预处理,包括光线吸收和散射的补偿以及基于最大后验盲解卷积,以提高图像质量。然后采用光线投射算法重建三维结构,实现三维图像平移、旋转、缩放、光线设置等操作。实验结果表明,该方法能够应用于普通配置计算机,可以处理各大品牌激光扫描共聚焦显微镜拍摄的图像序列,具有较快的处理速度和良好的人机交互等优点。
    Abstract: The methodology was proposed for image processing and interactive visualization of confocal microscopy images.Firstly,an intensity compensation algorithm was applied to reduce noises resulted from light absorption and scattering by objects and particles in the volume through which light passes.Secondly,a deconvolution algorithm based on maximum a posteriori was applied to improve the image stacks quality.At last,the ray-casting algorithm was used to reconstruct the fine details in the volume.Furthermore,virtual reality technique was integrated with volume rendering in order to provide users an intuitive interface to view the cells and its structures through translation,rotation and zoom operations.The speed and versatility of the program allows for convenient,fast,interactive examination of unknown cell structures even on common PC.The program can be applied for different types of confocal microscopy data sets with good results.
  • [1]

    SAKAS G,VICKER M G,PLATH P J.Case study:visualization of laser confocal microscopy datasets[A].Proceedings of the 7th IEEE Visualization Conference[C].San Francisco:IEEE Press,1996.375~379.

    [3]

    SUN Y L,RAJWA B,ROBINSON J P.Adaptive image-processingtechnique and effective visualization of confocal microscopy images[J].Microscopy Research and Technique,2004,64(2):156~163.

    [6]

    RAZDAN A,PATEL K,FARIN G E et al.Volume visualization of multicolor laser confocal microscope data[J].Computers & Graphics,2001,25(3):371~382.

    [7]

    FANG S F,DAI Y,MYERS F et al.Three-dimensional microscopy data exploration by interactive volume visualization[J].Scanning,2000,22(4):218~226.

    [8]

    RICHARD O D,PETER E H,DAVID G S.Pattern classification[M].2nd ed,New York:John Wiley & Sons Inc,2001.124~128.

    [9]

    YU D Y.Image processing and study on microscopy images[D].Nanjing:Nanjing University of Science and Technology,2002.23~35(in Chinese).

计量
  • 文章访问数:  5
  • HTML全文浏览量:  0
  • PDF下载量:  3
  • 被引次数: 0
出版历程
  • 收稿日期:  2006-08-15
  • 修回日期:  2006-11-05
  • 发布日期:  2007-10-24

目录

    /

    返回文章
    返回