Abstract: In order to collect photoacoustic signals and reconstruct images quickly,photoacoustic signal collecting and image reconstructing system based on virtual instrument is applied to collect photoacoustic signals.Then the program of MATLAB is used to process collected data by LABVIEW.The photoacoustic imaging of phantom is obtained with filtered back projection algorithm in the end.The resolution of reconstructed image is 0.15mm.The reconstructed image is corresponded well with phantom.The methodology and equipment show that this imaging system features high speed and convenience.Furthermore,it is likely to be developed into a cheaper and practical clinical diagnosis instrument.