Application of laser light scattering techniques in cell counting and classification
-
摘要: 系统地概述了近年来激光散射技术在血细胞计数及分类中应用的进展。介绍了多角度散射法、散射强度曲线法、两角度强度比值法、多向散射法、90°散射法,并对这些方法进行了评价。同时还论述了血细胞的理论散射模型。指出了需要解决的问题和研究发展方向。Abstract: In this paper,the development of laser light scattering technique applied in blood cells' classification and counting in recent years is reviewed.Methods of multiangle light scattering,light scattering curve,ratio of light scattering intensities,multidirection light scattering and right angle scattering are introduced and evaluated,respectively.The theoretical light scattering models of blood cells are discussed.Finally,the problems to be solved and the research directions are pointed out.
-
Keywords:
- aser light scattering /
- blood cell /
- counting /
- classification /
- laser flow cytometry
-
-
[1] Keren D F.Flow cytometry in clinical diagnosis.New York:A cademic Press,1989
[1] Nisyiya I,Cram L S,Gray J W.Flow cytometry and image analysis for clinical applications.Amsterdam,The Netherlands: Excerpta Medica,1991
[2] 武汉医学院第二附属医院检验科.实用临床医学检验.武汉:湖北人民出版社,1980 [3] 李树民,赵晓虹.动物学杂志,1988;23(3):36~38 [4] Cram L S,Martin J C,Steinkamp J A et al.Proc IEEE,1992;80(6):912~917
[5] Meyer R A,Brunsting A.Biophys J,1975;15:191~195
[6] Loken M R,Sweet R G,Herzenberg L A.Ann NY Acad Sci,1975;254:263~267
[7] Michael R L,Parks D R,Herzenberg L A.J Histochem Cytochem,1977;25(7):790~795
[8] Loken M R,St all A M.J Immunol Methods,1982;50:R85
[9] Bonner W A.Rev Scient Instrum,1972;43:404~501
[10] Mansberg H P.J Histochem Cytochem,1974;22:711~719
[11] Steinkamp J A,Hansen K M,Crisman H A.Rev Scient Insteum,1873;44:1301~1308
[12] Salzman G C,Crowell J M,Martin J C et al.Acta Cytol,1975;9(5):374~377
[13] Michael R L.J Histochem Cytochem,1986;34(1):284~291
[14] Salzman G C,Crowell J M,Goad C A et al.Clin Chem,1975;21(9):1297~1304
[15] Kerker M.The Scattering of Light and Other Electromagnetic Radiation.New York:Academic Press,1969
[16] Kaye P H,Ludlow I K,Milburn M R.J Coll Interface Sci,1980;70(3):167~171
[17] Jovin T M,Morris S J,Striker G et al.J Histochem Cytochem,1986;34(1):269~283
[18] Steinkamp J A.Rev Scient Instrum,1984;55:1375~1386
[19] Sloot P M A,Figdor C G.Appl Opt,1986;25(19):3559~3565
[20] Hoffman R A,Kung P C,Hansen W P et al.Proc Nat Acad Sci(U.S.A.),1980;77(8):4914~4917
[21] Ritchie A W S,Gray R A,Micklem H S.J Immunol Methods,1983;64:109~117
[22] Otten G R.Cytometry,1982;3:182~189
[23] Sloot P M A,Hoekstra A G,Hans van der Liet et al.Appl Opt,1989;28(10):1752~1761
[24] Van de Hulst H C.Light Scattering by Small Particles.New York:Wiley Press,1957
[25] Kattawar G W.Appl Opt,1967;6:1377~1382
[26] Kerker M,Cooke D D,Chew H et al.J O S A,1978;68:592~604
[27] Brunsting A,M ullaney P F.Appl Opt,1972;11:675~682
[28] Sloot P M A.Elast ic Light Scattering from Leucocytes in the Development of Computer Assisted Cell Separation.Ph.D. Thesis,Netherland Cancer Institute,1066 CX Amsterdlam,The Netherland,1988
[29] De Grooth B G,Terstappen L W M M,Puppels G J et al.Cytometry,1987;8:539~552
[30] Bickel W S,Watkins A J,Videen G.Biological particles as irregularly shaped scatterers.In:Schuerman D W ed.Light scattering by irregularly shaped particles,New York:Plenum,1980:299~305
计量
- 文章访问数: 5
- HTML全文浏览量: 0
- PDF下载量: 16