Enhancement of SNR and precision in measurement of time resolved fluorescence spectra
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摘要: 建立了随机噪音的经验模型,并通过计算机模拟超短寿命的荧光衰减过程,研究了利用取样法测量时间分辨荧光光谱实验中实验参数的选取对实验结果的影响。结果表明,增大“门宽”可以提高信噪比,从而实现微弱荧光信号的测量;减小“延时”可以提高时间分辨率,即提高测量精度;选取不同的“门宽”时,测量结果中各个衰减组分初始荧光强度的比例关系会发生改变,并推导出测量值与实际值的对应关系,可以从测量结果反推出实际的比例。另外,测量了GaP样品的时间分辨荧光光谱,实验结果验证了所提出的随机噪音经验模型以及提高信噪比与测量精度的方法。并且利用该结论测量了LN:Er样品微弱荧光的时间分辨荧光光谱。Abstract: An experiential model of random noise is setup to simulate the ultrafast fluorescence decay for researching how the experiment parameters influence the measurement of time resolved fluorescence spectra.It is found that the SNR can be improved extremely by increasing the gating time and precisi on can be improved by decreasing the delaying time.So the feeble fluorescence signal can be measured and the time resolving degree can be enhanced.The ratio between each decay component's initial intensity varies with different gating time.The relationship between them has been found and the actual value can be deduced from measurement result.The experiment results of GaP testifies our model and the simulation conclusion.This method is successfully used to measure the time resolved fluorescence spectra of LN:Er whose fluorescence is weak.
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Keywords:
- time resolved fluorescence spectra /
- sampling method /
- SNR /
- precision
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