Analysis of Ar+ laser-induced erythrocyte fluorescencespectra with various wavelengths
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摘要: 根据对可见波段、不同波长的Ar+激光激发同一浓度红细胞溶液产生荧光光谱的研究,发现从600nm到860nm较大的范围内红细胞均存在较为丰富的荧光光谱谱峰,且在波长为620nm附近的谱峰随激发光波长的变化而变化。通过理论分析得到,激光诱导的红细胞荧光光谱主要是红细胞膜上的细胞色素基团、双磷脂分子等以及红细胞中的血红蛋白大分子中的血红素基团等构成的多种荧光团作用的结果,且在620nm附近的谱峰不仅含有荧光光谱成分,还有喇曼散射的光谱成分。研究结果可对低功率激光诱导生物组织荧光光谱技术在医疗中的应用有参考意义。Abstract: Based on the investigation of Ar+laser-induced erythrocyte fluorescence spectra with various visible wavelengths under certain concentration of erythrocyte,it is found that there are plentiful peaks in the fluorescence spectra from 600nm to 860nm,and the peaks near 620nm change with the variation of exciting light wavelength.The results indicate that laser-induced erythrocyte fluorescence spectra are mainly due to numerous fluorophores from part thereof cytochrome,and pair-phosphatide on the erythrocyte membrane and the part thereof haem from the hemoglobin.In addition,there are not only laser excited erythrocyte fluorescence peaks but also the Raman scattered light by the erythrocyte in the near 620nm.The investigation may be useful for low lever laser induced tissue fluorescence spectra applied in medical treatment.
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Keywords:
- Ar+laser /
- fluorescence spectra /
- erythrocyte /
- fluorophore
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