Abstract: The laser confocal scanning microscopic (LCSM) system is one kind of modern microscopic instruments which are characterized by depth discrimination capability. However,in order to obtain the three-dimensional images of an object,the three-dimension reconstruction is very importment. In this paper,the theories and methods of how to create accurate depictions of three-dimension data on the screen of a computer device is discussed and a new three-dimension reconstruction method based on surface is proprosed.The experiment results show that the reconstructed images have high quality.