Abstract: The fluorescence spectra of alcohol solution induced by ultraviolet light and its characteristic are studied experimentally and theoretically in this paper. The results show that alcohol solution can emit strong fluorescence at 395nm when excited by ultraviolet light with the wavelength of 254nm.It is considered that this fluorescence comes from the transition from n→σ* of the lone-pair electrons in the hetero atom group (C-OH) of the ethanol molecule. Investigation on the fluorescence spectra and its characteristics will contribute to study of other organic macromolecule fluorescence spectra when alcohol serves as solute.