Abstract: Based on the multiplex holography with rotating prism and one step rainbow holography,a multiplex one-step rainbow holography,a multiplex one-step rainbow holography with r otat ing prism is presented. Its principle is discussed in detail.It is possible to record many rainbow holograms by rotating prism and multiple exposure way with ordinary He-Ne laser on single holographic plate,and reconstruct with white light at the same time.The experimental results conform to theoretical analysis.