Research on laser scanning confocal fluorescent microscopic system
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摘要: 讨论了激光扫描共焦荧光显微系统的光学设计问题,成象机理,参数确定及其设计关键,介绍了高精度光学扫描器件的设计方法及实现荧光共焦成象中成象器件的优化设计.Abstract: The laser scanning confocal fluorescent microscope has the feature of high longitudinal resolution power and can provide clear 2-dimension or 3-dimension medical image.The purpose of this paper is to introduce the optical design,imaging principles and key points of design of the laser scanning confocal fluorescent microscope system.To achieve high quality imaging,it is important to realize the precision laser optical scanner and optimize the design of imaging components.
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Keywords:
- laser scanning /
- confocal /
- microscopy /
- optical design
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[1] Gu M.Sheppard C J R,Joural of M odern Optics,1991;38(11)
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