Abstract: In order to improve the effect of laser technology in the treatment of pigmentary diseases and other biomedical application, 1064nm/ 532nm/ 570nm three-wavelength lasers were developed. Electro-optic Q-switched Nd:YAG laser was used to obtain 1064nm pulse laser with narrow pulse width of 11ns. Output of 532nm laser was obtained by using KTP non-linear crystal for optical extra-cavity frequency doubling. With solid dye block as laser gain medium and frequency doubling light as pump light, yellow light output with a central wavelength of 570nm was obtained, and the light-to-light conversion efficiency was 61.3%. The results show that, by changing the injection voltage of xenon lamp, output characteristics of 1064nm laser pulse can be adjusted. By increasing the cavity length of the solid dye laser, output spectral characteristics of the dye laser can be adjusted. The research results are of great significance to the flexible application of lasers.