Abstract: In order to investigate laser disturbing effects under different incident angles, experimental study and theoretical analysis were carried out by gradually adjusting the optical axis pointing. Spot positions, receiving energies and spot shapes of laser on the detector were achieved under different incident angles. The results show that spot positions shift linearly with the increase of incident angle. The spot would deviate 16pixel from the center of detector with the increase of 0.1° of incident angle. Receiving energy decreases with the increase of incident angle and the reduction is less than 1% for the optical system with viewing angle of 8°. Spot shape meets space shift invariance under different incident angles. The study is helpful for the experiment of laser jamming electro-optical imaging system.