Abstract: In order to prepare Yb3+-doped silicate laser glass and analyze its laser characteristics, based on the no-chemical vapor deposition high temperature melting process in an oxygen-enriched atmosphere, the data of the absorption and fluorescence spectra of the glass were obtained according to Fuchbauer-Ladenburger(F-L) method. It can be concluded that there are board-band absorption peaks in the absorption band of 850nm~1100nm near-infrared wavelengths. The main absorption peak is at 978nm and the sub-peak value is at 919nm respectively, the main fluorescent peak is at 1018nm with the sub-peak value at 970nm. The integrated absorption cross section, stimulated emission cross section and fluorescent lifetime of Yb3+ were calculated theoretically. This result is useful to characteristic analysis of silicate laser glass. The comprehensive test results show that they can meet the application requirements of laser glass.