Abstract: To study the influence of temperature change on carbon monoxide absorption spectral lines,at first,the temperature-relative parameters,such as carbon monoxide absorption spectral lines intensity,comprehensive widened line shape function and absorption coefficients were obtained based on high-resolution transmission molecular absorption database.Then starting from absorption spectrum principle,the temperature-relative parameters were simulated with MATLAB and the temperature-relative curves were drawn.The results show that the effect of temperature on carbon monoxide absorption spectral lines,especially on the comprehensive widened line shape function,is complex and that different laser frequencies also affect the relationship of temperature and comprehensive widened line shape function and the absorption coefficients,which gives some advice for the absorption and measurement of carbon monoxide in practical application.