Abstract: In order to understand the effect of the system factors,such as light intensity,magnification,CCD and background,on the reconstructed image of different diameter particles in the process of holographic reconstruction,a series of experiments were performed.The experimental results showed that,by adjusting the light intensity and making the background light intensity closed to the lower limit of CCD threshold,images with smoothing background and high definition were acquired.For particles with diameter less than 20μm and a reconstruction system with a certain magnification,a little higher intensity of reconstruction light was needed for ideal images.It was favorable for image keeping the experimental environment dark.Results show that high quality particle images can be obtained by means of the above methods in the process of particle holographic reconstruction.