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Volume 28 Issue 2
Sep.  2013
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Study on optimal culturing of high proteinase-producing mucor racemosus mutated by CO2 laser

  • 1.

    College of Life Science, Sichuan University, Chengdu 610064, China

  • Received Date: 2003-08-11
    Accepted Date: 2003-11-20
  • Mucor racemosus 137 was isolated from fermented bean curd and brought to mutation by radiation of CO2 laser. The duration for radiation was 40s with distance of 22cm, and the diameter of laser beam was 0.4cm. High proteinase-producing mutant MR137-3 was obtained after screening cultures. The MR137-3 was found to have stable proteinase-producing activity in descendant cultures. The optimal culturing could be obtained at 30℃, pH6.0, culturing duration of 72h, with water content of 65% of culture medium.
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  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

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Study on optimal culturing of high proteinase-producing mucor racemosus mutated by CO2 laser

  • 1. College of Life Science, Sichuan University, Chengdu 610064, China
  • Received Date: 2003-08-11
  • Accepted Date: 2003-11-20
  • Available Online: 2004-03-25

Abstract: Mucor racemosus 137 was isolated from fermented bean curd and brought to mutation by radiation of CO2 laser. The duration for radiation was 40s with distance of 22cm, and the diameter of laser beam was 0.4cm. High proteinase-producing mutant MR137-3 was obtained after screening cultures. The MR137-3 was found to have stable proteinase-producing activity in descendant cultures. The optimal culturing could be obtained at 30℃, pH6.0, culturing duration of 72h, with water content of 65% of culture medium.

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