Apoptosis of human bladder cancer BIU-87 cells after photodynamic therapy with laser-activated BPD-MA determined with TUNEL assays
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1.
Institute of Ultrasound Imaging, Chongqing University of Medical Sciences, Chongqing 400010, China;
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2.
Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;
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3.
Department of Laser Research, Rehabilitation Center, Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, China;
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4.
Center of Plastic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
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Received Date:
2004-12-16
Accepted Date:
2005-01-25
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Abstract
In order to study the mechanism of photodynamic therapy on malignant tumors,photosensitization of BPD-MA is activated by laser with red light(632.8nm) delivered at 10mW/cm2to give a total dose of 2.4J/cm2.Apoptosis of human bladder cancer BIU-87 cells is determined with flow cytometry with PI staining and terminal deoxyuridine nick end labeling(TUNEL) assays.Results show that photodynamic therapy with BPD-MA significantly increases the rate of apoptosis in BIU-87 cells.A lot of the positive cells in which their nucleus is brown-yellow fine particle shape or overflowing-dispersed are observed after treatment of cells with photodynamic therapy with BPD-MA.However,laser irradiation alone,BPD-MA alone and sham radiation without BPD-MA can not affect apoptosis of human bladder cancer BIU-87 cells.A conclusion can be made that treatment of cells with photodynamic therapy obviously induces human bladder cancer BIU-87 cells to apoptosis,which may be one of the important mechanism of photodynamic therapy on malignant tumors.
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