Analization of blood cells fluorescence spectra induced by LED at the wavelength of 407nm

Gao Shumei; Luo Xiaosen; Shen Jian; Lu Jian; Ni Xiaowu

The fluorescence spectra for whole blood,erythrocyte and hemoglobin from healthy and fresh experimental mice under the wavelength of 407nm (Δλ1/2≈18nm) excitation are measured by a grating spectrometer,and their emitting mechanism and spectral characteristic are investigated. The experimental results and theoretic analization about it are presented in this paper. It shows that the blood or erythrocyte emitting fluorescence quantum yield efficiency under 407nm-LED excitation may reach above 90%,the blood fluorescence mainly result from fluorophores of the erythrocyte,but fluorescence spectra of hemoglobin by 407nm-LED excitation are obviously different from that of erythrocyte,and the fluorescence quantum yield of cracked erythrocyte remarkably declines and its spectral profiles are being distorted with the consistency of the cracked erythrocyte increasing.

1. Department of Physics, Xuzhou Normal University, Xuzhou, 221009;

2. Department of Applied Physics, Nanjing University of Science and Technology, Nanjing, 210094;

3. Nanjing Medical College, Second Military Medical University, Nanjing, 210099

Received Date: 2002-01-15

Accepted Date: 2002-04-28

Available Online: 2002-11-25

Abstract: The fluorescence spectra for whole blood,erythrocyte and hemoglobin from healthy and fresh experimental mice under the wavelength of 407nm (Δλ1/2≈18nm) excitation are measured by a grating spectrometer,and their emitting mechanism and spectral characteristic are investigated. The experimental results and theoretic analization about it are presented in this paper. It shows that the blood or erythrocyte emitting fluorescence quantum yield efficiency under 407nm-LED excitation may reach above 90%,the blood fluorescence mainly result from fluorophores of the erythrocyte,but fluorescence spectra of hemoglobin by 407nm-LED excitation are obviously different from that of erythrocyte,and the fluorescence quantum yield of cracked erythrocyte remarkably declines and its spectral profiles are being distorted with the consistency of the cracked erythrocyte increasing.

HTML

Reference (14)

[1]	李静,李世荣,高革 et al.中华肿瘤杂志,2000,22(5):893～993.
[2]	金曼文,宋支才.激光杂志,1991,12(3):148～150,131.
[3]	刘文清, Ram R.科学通报,1989,34(9):700～703.
[4]	赵箭光,杨佩青.中国医学物理学杂志,1996,13(4):220～222.
[5]	Kessel D.IEEE J Q E,1987,QE23(10):1718～1720.
[6]	Tiina I. IEEE J Q E,1987,QE23(10):1703～1717.
[7]	Kertesz I,Feny M,Mester E et al.Opt Laser Technol,1982,14:31～32.
[8]	朱平主编.低强度激光血管内照射疗法,南宁:广西科学技术出版社,1999.
[9]	兰秀凤,刘晓然,王亚伟 et al.中国激光,2000,27 (5):477～480.
[10]	陈荣,陈艳娇,杨文琴 et al.激光生物学报,1999,8(1):62～65.
[11]	Niemz M H.Laser-tissue interactions fundamentals and applications.Heidelberg:Springer-Verlag Berlin,1996.
[12]	林克椿主编.生物物理学.武汉:华中师范大学出版社,1999.
[13]	Udenfriend S.Fluorescence assay in biology and medicine,New York: Academic,1962.
[14]	Fisher F,Arnold J R P. Instant notes in chemistry forbiologists.BIOS Scientific Publishers Limited,1999.

Analization of blood cells fluorescence spectra induced by LED at the wavelength of 407nm

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views