Study on electronic control system for laser scanning confocal fluorescence microscope

The electronic control system for laser scanning confocal fluorescence microscope is described in this paper.For improvement of the positing precision of laser scanner,a high accuracy scanning image sampling method,based on pixels clock generator,is adapted to correct the non-linear output image pincushion distortion caused by the rotational inertial of the e mechanical parts of laser scanner.In order to detect the scanning spatial position under the case of step motor driving,according to the experimentally measured I-θ curve,we can get the phase current wave form which compensates the non-linearity of I-θ curve by means of interpolation method.Therefore,the step motor can operate with high angle uniformity under the condition of low current mode.