脐带血与先天性心脏病患儿红细胞的光谱分析

WU Zhi-hui; CUI Xiang-rong; HUANG Dai-zheng; CHEN Chao-wang; MO Hua

doi:10.3969/j.issn.1001-3806.2012.02.024

Volume 36 Issue 2

Jan. 2012

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脐带血与先天性心脏病患儿红细胞的光谱分析

1.
Department of Physics, Guangxi Medical University, Nanning 530021, China;
2.
First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China

Corresponding author: MO Hua, mohuawuli@vip.sing.com
Received Date: 2011-06-24
Accepted Date: 2011-08-30

Abstract

为了探讨脐带血红细胞和先天性心脏病患儿红细胞的内容物的改变情况及其变化机理,为先天性心脏病的产前诊断提供有用信息,采用激光光镊喇曼光谱系统,测定了脐带血红细胞与先天性心脏病患儿红细胞的喇曼光谱,通过对两者喇曼光谱进行比较分析,发现脐带血红细胞与先天性心脏病患儿红细胞的光镊喇曼光谱有较大的差异。与脐带血红细胞相比,先天性心脏病患儿红细胞的整体谱线偏弱,部分的特征谱线发生位移。该研究结果将为提高胎儿先天性心脏病的检出率提供一种新的、快速简便的光谱分析手段,为临床诊断提供有力的实验依据。
- medical optics and biotechnology,
- Raman spectra,
- optical tweezers,
- red blood cells,
- umbilical cord blood,
- congenital heart disease

References

[1]	CHOTTARD G,KAZANSKAYA I,BRUSCHI M.Resonance Raman study of multihemic c-type cytochromes from desulfuromons acetoxidans[J].Chemistry,2000,A267(4):1050-1058.
[2]	FEOFANOV A V,GRICHINE A I,SHITOVA L A,et al.Confocal Raman micro spectroscopy and imaging study of theraphthal in living cancer cells[J].Biophysical Journal,2000,7(1):499-512.
[3]	CHENG J X,KEVIN J Y,ZHENG G F,et al.Laser-scanning coherent anti-Stokes raman scattering microscopy and applications to cell biology[J].Biophysical Journal,2002,83(1):502-509.
[4]	LAI J Z,WANG G W,ZHAO C G,et al.Analysis of the Raman spectrum of single tricholoma lobayense spores trapped by laser tweezers[J].Chinese Journal of Spectroscopy Laboratory,2010,27(2):545-549(in Chinese).
[5]	AI M,LIU J X,YAO H L.Raman spectra of single reticulocytes and small lymphocytes in blood[J].Acta Optica Sinica,2008,28(1):1-6(in Chinese).
[6]	YUE L Y,WANG G W,YAO H L,et al.Study of Raman spectroscopy of single human red blood cell trapped optically after thawed[J].Acta Biophysica Sinica,2007,23(2):75-80(in Chinese).
[7]	YANG W P,YAO H L,ZHU M.Raman spectrums of mono-hepatocellular carcinoma[J].Laser & Infrared,2007,37(9):824-827(in Chinese).
[8]	YAO H L,ZHU M,WANG G W,et al.Study of Raman spectra of single carcinoma of nasopharynx cell[J].Spectroscopy and Spectral Analysis,2007,27(9):1761-1764(in Chinese).
[9]	TANG H Y,YAO H L,WANG G W.NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers[J].Optics Express,2007,15(20):12708-12716.
[10]	WOOD B R,CASPERS P,PUPPELS G J.Resonance Raman spectroscopy of red blood cells using near-infrared laser excitation[J].Analytical and Bioanalytical Chemistry,2007,387(5):1691-1703.
[11]	OJEDA J F,XIE C A,LI Y Q,et al.Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy[J].Optics Express,2006,14(12):5385-5393.
[12]	XIE C A,CHEN D,LI Y Q.Raman sorting and identification of single living micro-organisms with optical tweezers[J].Optics Letters,2005,30(14):1800-1802.
[13]	NAN N,SHAO Y H,JIANG Y L,et al.Study of resonance raman detection of carotenoid in human[J].Laser Technology,2008,32(5):490-492(in Chinese).
[14]	ASHKIN A,SCHUTZE K,DZIEDZIC J M.Force generation of organelle transport measured in vivo by an infrared laser[J].Nature,1990,348(6299):346-348.
[15]	XIE C A,LI Y Q.Confocal micro-Raman spectroscopy of single biological cell using optical trapping and shifted excitation deference techniques[J].Journal of Applied Physics,2003,93(5):2982-2986.
[16]	XIE C A,MACE J,DINNO M A.Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy[J].Analytical Chemistry,2005,77(14),4390-4397.
[17]	WU D J,HONG Y,BU M,et al.A model for light scattering from red blood cells[J].Laser Technology,2007,31(2):156-159(in Chinese).
[18]	WU Z H,MO H,HUANG D Zh,et al.Red blood cells in patients with arrhythmias using optical tweezers Raman spectroscopy[J].Laser & Optoelectronics Progress,2011(2):021701(in Chinese).
[19]	ZHAI Y L,LIU X C,LIU F Q,et al.Analysis on erythrocyte aggregation and deformation in cord blood and different age of adult[J].Chinese Journal of Microcirculation,1999,9(2):27-28(in Chinese).
[20]	ZHOU A Q.Thinking and selection of methods in diagnosis of congenital heart disease[J].Journal of Diagnostics Concepts & Practice,2006,3(5):276-278(in Chinese).
[21]	XU Y M.Raman spectroscopy and its application on structural biology[M].Beijing:Chemical Industry Press,2005:11-35(in Chinese).
[22]	CHEN X L,WANG G W,LIU J X,et al.Technique and advance of Raman spectroscopy on functional erythrocyte study[J].Acta Laser Biology Sinica,2008,17(6):845-852(in Chinese).
[23]	WANG M,YU F,LONG Q.The application and development of Raman spectroscopy on protein conformation[J].Acta Laser Biology Sinica,2007,16(4):516-520(in Chinese).

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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脐带血与先天性心脏病患儿红细胞的光谱分析

Corresponding author: MO Hua, mohuawuli@vip.sing.com

1. Department of Physics, Guangxi Medical University, Nanning 530021, China;

2. First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China

Received Date: 2011-06-24

Accepted Date: 2011-08-30

Available Online: 2012-03-25

Abstract: 为了探讨脐带血红细胞和先天性心脏病患儿红细胞的内容物的改变情况及其变化机理,为先天性心脏病的产前诊断提供有用信息,采用激光光镊喇曼光谱系统,测定了脐带血红细胞与先天性心脏病患儿红细胞的喇曼光谱,通过对两者喇曼光谱进行比较分析,发现脐带血红细胞与先天性心脏病患儿红细胞的光镊喇曼光谱有较大的差异。与脐带血红细胞相比,先天性心脏病患儿红细胞的整体谱线偏弱,部分的特征谱线发生位移。该研究结果将为提高胎儿先天性心脏病的检出率提供一种新的、快速简便的光谱分析手段,为临床诊断提供有力的实验依据。

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Reference (23)

[1]	CHOTTARD G,KAZANSKAYA I,BRUSCHI M.Resonance Raman study of multihemic c-type cytochromes from desulfuromons acetoxidans[J].Chemistry,2000,A267(4):1050-1058.
[2]	FEOFANOV A V,GRICHINE A I,SHITOVA L A,et al.Confocal Raman micro spectroscopy and imaging study of theraphthal in living cancer cells[J].Biophysical Journal,2000,7(1):499-512.
[3]	CHENG J X,KEVIN J Y,ZHENG G F,et al.Laser-scanning coherent anti-Stokes raman scattering microscopy and applications to cell biology[J].Biophysical Journal,2002,83(1):502-509.
[4]	LAI J Z,WANG G W,ZHAO C G,et al.Analysis of the Raman spectrum of single tricholoma lobayense spores trapped by laser tweezers[J].Chinese Journal of Spectroscopy Laboratory,2010,27(2):545-549(in Chinese).
[5]	AI M,LIU J X,YAO H L.Raman spectra of single reticulocytes and small lymphocytes in blood[J].Acta Optica Sinica,2008,28(1):1-6(in Chinese).
[6]	YUE L Y,WANG G W,YAO H L,et al.Study of Raman spectroscopy of single human red blood cell trapped optically after thawed[J].Acta Biophysica Sinica,2007,23(2):75-80(in Chinese).
[7]	YANG W P,YAO H L,ZHU M.Raman spectrums of mono-hepatocellular carcinoma[J].Laser & Infrared,2007,37(9):824-827(in Chinese).
[8]	YAO H L,ZHU M,WANG G W,et al.Study of Raman spectra of single carcinoma of nasopharynx cell[J].Spectroscopy and Spectral Analysis,2007,27(9):1761-1764(in Chinese).
[9]	TANG H Y,YAO H L,WANG G W.NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers[J].Optics Express,2007,15(20):12708-12716.
[10]	WOOD B R,CASPERS P,PUPPELS G J.Resonance Raman spectroscopy of red blood cells using near-infrared laser excitation[J].Analytical and Bioanalytical Chemistry,2007,387(5):1691-1703.
[11]	OJEDA J F,XIE C A,LI Y Q,et al.Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy[J].Optics Express,2006,14(12):5385-5393.
[12]	XIE C A,CHEN D,LI Y Q.Raman sorting and identification of single living micro-organisms with optical tweezers[J].Optics Letters,2005,30(14):1800-1802.
[13]	NAN N,SHAO Y H,JIANG Y L,et al.Study of resonance raman detection of carotenoid in human[J].Laser Technology,2008,32(5):490-492(in Chinese).
[14]	ASHKIN A,SCHUTZE K,DZIEDZIC J M.Force generation of organelle transport measured in vivo by an infrared laser[J].Nature,1990,348(6299):346-348.
[15]	XIE C A,LI Y Q.Confocal micro-Raman spectroscopy of single biological cell using optical trapping and shifted excitation deference techniques[J].Journal of Applied Physics,2003,93(5):2982-2986.
[16]	XIE C A,MACE J,DINNO M A.Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy[J].Analytical Chemistry,2005,77(14),4390-4397.
[17]	WU D J,HONG Y,BU M,et al.A model for light scattering from red blood cells[J].Laser Technology,2007,31(2):156-159(in Chinese).
[18]	WU Z H,MO H,HUANG D Zh,et al.Red blood cells in patients with arrhythmias using optical tweezers Raman spectroscopy[J].Laser & Optoelectronics Progress,2011(2):021701(in Chinese).
[19]	ZHAI Y L,LIU X C,LIU F Q,et al.Analysis on erythrocyte aggregation and deformation in cord blood and different age of adult[J].Chinese Journal of Microcirculation,1999,9(2):27-28(in Chinese).
[20]	ZHOU A Q.Thinking and selection of methods in diagnosis of congenital heart disease[J].Journal of Diagnostics Concepts & Practice,2006,3(5):276-278(in Chinese).
[21]	XU Y M.Raman spectroscopy and its application on structural biology[M].Beijing:Chemical Industry Press,2005:11-35(in Chinese).
[22]	CHEN X L,WANG G W,LIU J X,et al.Technique and advance of Raman spectroscopy on functional erythrocyte study[J].Acta Laser Biology Sinica,2008,17(6):845-852(in Chinese).
[23]	WANG M,YU F,LONG Q.The application and development of Raman spectroscopy on protein conformation[J].Acta Laser Biology Sinica,2007,16(4):516-520(in Chinese).

脐带血与先天性心脏病患儿红细胞的光谱分析

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views