Characteristics and composition analysis of pure liquid milk based on 3-D fluorescent spectra
-
Corresponding author:
GAO Shu-mei, gaosm@jiangnan.edu.cn
-
Received Date:
2011-03-02
Accepted Date:
2011-04-11
-
Abstract
To detect the quality of pure milk rapidly, its spectral characteristics were analyzed combining 3-D fluorescent spectra with multi-peak fitting method, and its main compositions were obtained. The results show that its excitation spectrum ranges from 250nm to 310nm, and the peak wavelength is 290nm. The main emission spectrum ranges from 280nm to 500nm with the peak adjacent to 330nm.Meanwhile, the fluorescent spectrum has five spectral peaks(Ⅰ~Ⅴ), where peakⅠis generated by some proteins under the influence of lactose, peak Ⅱis generated by fat and vitamin E jointly, peak Ⅲ is generated by protein directly, peak Ⅳ is generated by some proteins under some unknown influence, and peak Ⅴ is generated jointly by microelements including vitamin A, vitamin B1, and B6. The study results provide some basic principles for studying milk composition with fluorescent spectroscopy.
-
-
References
[1]
|
HE Y C.Development of Chinese dairy industry[M].Beijing:Science Press,2010:63-78(in Chinese). |
[2]
|
ZHANG X L,HUA R,YANG Q L,et al.Hand-held extinction photometer for the detection of idntegrate quality of milk on-site rapidly[J].Analytical Chemistry,2009,37(s1):45(in Chinese). |
[3]
|
LLU J,YU C Q,LI J Z,et al.Study on the deteriorating course of fresh milk by laser-induced fluorescence spectra[J].Spectroscopy and Spectral Analysis,2001,21(6):769-771(in Chinese). |
[4]
|
CHEN X,GU R,CHEN X,et al.Research on feature extractions of 3-D spectrum of milk[J].China Dairy Industry,2009,37(7):13-15(in Chinese). |
[5]
|
LU J,GAO Sh M,XIONG J,et al.Characteristics and mechanism for fluorescence spectroscopy of female urine[J].Laser Technology,2010,34(1):45-47(in Chinese). |
[6]
|
LI Y L,DENG L Q,GUO X M,et al.Determination of vitamin E in vegetable by synchronous fluorescence spectrometry[J].Hebei University (Natural Science Edition),2009,29(4):412-415 (in Chinese). |
[7]
|
ZHAO W Y,YANG C F,LIU Y.A study on spectral characteristics of saturated fatty acids-ethanol solution[J].Xuzhou Normal University (Natural Science Edition),2009,27(2):55-58(in Chinese). |
[8]
|
ZHAO N M,ZHOU H M.Biophysics[M].Beijing:Higher Education Press,2000:300-302(in Chinese). |
[9]
|
GU L L,WANG K,WANG M,et al.Fluorescence study on the interaction of galactose and β-galactosides with a galectin from the edible mushroom agrocybe aegerita[J].Analytical Science,2006,22(6):621-625(in Chinese). |
[10]
|
WU G H,HE C Y.Application of artificial neural network to simultaneous spectrofluorimetric determination of vitamin B1,B2 and B6[J].Spectroscopy and Spectral Analysis,2003,23(3):535-538(in Chinese). |
[11]
|
ZAIDI F,ROUISSI H,DRIDI S,et al.Front-face fluorescence specctroscopy as a rapid and non-destructive tool for differentiating between Sicilo-Sarde and Comisana ewe's milk during lactation period:a preliminary study[J].Food & Bioprocess Technology,2008,1(2):143-151. |
-
-
Proportional views
-