Photography measurement of optical parameters of biological tissues
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1.
Science College, Civil Aviation University of China, Tianjin 300300, China;
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2.
Key Laboratory of Opto-Electronic Information Science and Technology of Ministry of Education, Tianjin University, Tianjin 300072, China
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Received Date:
2009-12-22
Accepted Date:
2010-03-18
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Abstract
To measure optical parameters of biological tissues,CCD diffusing reflection and transmission measurements were introduced.Analyzing the CCD photograph of diffusing reflection distribution on the specimen surface,the reduced scattering coefficient and absorption coefficient of biological tissue simulating liquid (intralipid-20% diluents),beef,pork and chicken were derived from nonlinear regression of the diffusion equation with spatially resolved steady-state diffuse reflectance which was measured by the CCD diffusing reflection measuring equipment.The scattering coefficient was derived from linear regression of the Beer-Lambert equation with transmission light intensity of biological tissue simulating liquid of different titer which was measured by the CCD transmission measuring equipment,and then the reduced scattering coefficient was also deduced.Optical parameters of the same specimen measured by CCD diffusing reflection and transmission measurements were compared with those measured by others,and they were coherent.It is shown that the experimental CCD equipment and the treatment methods used to measure optical parameters have a high accuracy.
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References
|
[1]
|
PATTERSON M S,WILSON B C,WYMAN D R.The propagation of optical radiation in tissue.Ⅱ:Optical properties of tissues and resulting fluence distributions[J].Lasers in Medical Science,1991,6 (4):379-390. |
|
[2]
|
LI Zh M,LIU X H.The theoretical analysis of thermal interaction between laser and bio-tissue[J].Journal of Optoelectronics· Laser,2002,13 (8):860-863 (in Chinese). |
|
[3]
|
LAI J Ch,LI Zh H,WANG Ch Y,et al.Light transmitting properties of muscle tissues and modification of radiative transfer theory[J].Laser Technology,2007,31(3):265-280 (in Chinese). |
|
[4]
|
SPLINTER R,NNANEY G A,LITTMAN L,et al.Monitoring tissue optical characteristics in situ using a CCD camera[J].Lasers in the Life Sciences,1994,6(1):15-25. |
|
[5]
|
BOLT R A,ten BOSCH J J.Method for measuring position-dependent volume reflection[J].Appl Opt,1993,32(24):4641-4645. |
|
[6]
|
KIENLE A,LILGE L,PATTERSON M S,et al.Spatially resolved absolute diffuse reflectance measurements for noninvasive determination of the optical scattering and absorption coefficients of biological tissue[J].Appl Opt,1996,35(13):2304-2314. |
|
[7]
|
XIE Sh S.HUANG L H,ZHENG W,et al.Measurement of the optical properties of mammalian tissues in vitro[J].Optical Journal,1996,25(2):97(in Chinese). |
|
[8]
|
KIENLE A,PATTERSON M S.Improved solutions of the steady-state and the time-resolved diffusion equations for reflectance from a semi-infinite turbid medium[J].J O S A,1997,14(1):246-254. |
|
[9]
|
ISHIMARU A.Wave propagation and scattering in random media[M].New York:Academic Press,1978:142-157. |
|
[10]
|
HASKELL R C,SVAASAND L O,TSAY T T,et al.Boundary conditions for the diffusion equation in radiactive transfer[J].J O S A,1994,11(10),2727-2741. |
|
[11]
|
van STAVEREN H J,MOES C J M,van MARIE J,et al.Light scattering in intralipid-10% in the wavelength range of 400-1100nm[J].Appl Opt,1991,30(31):4507-4514. |
|
[12]
|
XU H P,PATTERSON M S.Determination of the optical properties of tissue-simulating phantoms from interstitial frequency domain measurements of relative fluence and phase difference[J].Optics Express,2006,14(14):6485-6501. |
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