Fluorescence spectra of methyl parathion and its characteristic

BI Lin-na; CHEN Guo-qing; WANG Jun; YAN Hao-ran

doi:10.3969/j.issn.1001-3806.2010.02.030

Volume 34 Issue 2

May 2010

Article Contents

Turn off MathJax

Article Navigation > LASER TECHNOLOGY > 2010 > 34(2): 253-257

Citation:

Fluorescence spectra of methyl parathion and its characteristic

1.
School of Science, Jiangnan University, Wuxi 214122, China

Corresponding author: CHEN Guo-qing, cgq2098@163.com ;
Received Date: 2008-12-08
Accepted Date: 2009-03-05

Abstract

In order to study the fluorescence spectra of methyl parathion and its characteristics, the spectral characteristics of standard methyl parathion solution induced by UV-light were experimentally studied with a multifunctional spectrometer system.Based on theoretical analysis by means of GAUSSIAN software and the methods of quantum mechanics,the mechanism of the fluorescence spectra of standard methyl parathion solution and the transition energy of methyl parathion were obtained and the transition energy of the linear structure fluorophore was estimated. The experimental valve was a little different from the theoretical value.The results show that there are four fluorescence peaks generated when the standard methyl parathion solution is excited by UV light at the wavelength of 200nm-320mn. The peak position is located at 435nm, 467nm, 567nm and 650nm respectively. The best excitation wavelength is 200nm and 295nm. The four fluorescence peaks are generated by the combined actions of the methyl parathion and the acetone.In methyl parathion molecule, there are the transition of n electrons in nitryl, the transition of electronic structure formed by the oxygen atom and the benzene ring and the transition of n electrons in P=S group.In acetone molecule, there is the transition of n electrons in C=O group. These results are helpful for testing organophosphorus pesticide's content in the area such as food safety inspection.
- spectroscopy,
- fluorescence characteristic,
- fluorescence analysis,
- organophosphorus pesticide,
- molecular structure,
- energy level,
- food safety

References

[1]	YANG Y L,LIU H L.Application study of fluorescent analysis on pesticide residue detection[J].Life Science Instruments,2007,5(9):9-13(in Chinese).
[2]	DENG Y H.To detect trace organic phosphorus pesticide using GC-pusle FPD[J].Administration and Technique of Environmental Monitoring,2003,15(3):24-26(in Chinese).
[3]	SU J F,LIN G Y,CHEN J X,et al.Determination of residual cumyluron in vegerable by solid phase extraction with gas chromatography/mass spectrometry and its fragmentation mechanism study[J].Chinese Journal of Analytical Chemistry,2008,36(4):545-548(in Chinese).
[4]	HE A A,ZHAO J,DONG X H,et al.Determination of residual carbendazim,thiabendazile,imidacloprid and acetamiprid in apple and apple juice concentrate by HPLC[J].Chinese Journal of Analysis Laboratory,2008,27(6):64-67(in Chinese).
[5]	XU X L,LI L,DING G D,et al.Rapid analysis of 13 pesticide residues in rice by large volume injection/gas chromatography-mass spectrometry coupled with solid phase extraction[J].Journal of Instrumental Analysis,2008,27(1):30-33(in Chinese).
[6]	ZHAO Y Y,LIU H F,HAO L,et al.Determination of paraquat in serum by ultraviolet spectrophotometry[J].Journal of Environment and Health,2007,24(5):346-347(in Chinese).
[7]	JACKSON P.Advantages of combined gas chromatograph-mass spectrometry-matrix isolation fourier transform infrared analysis[J].Analytical Proceeding,1993,30(10):135-139.
[8]	QIU Zh,LU J F,HE Y.Rapid detection system development and influence factor analysis for pesticide residues in agricultural products by enzyme inhibition method[J].Transactions of the Chinese Society of Agricultural Engineering,2007,23(9):229-233(in Chinese).
[9]	ROLFD H S.Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction[J].Analytical and Bioanalytical Chemistry,2002,372(2):268-272.
[10]	WU M,DING J H,WANG Ch J.Study on the polarization fluorescence spectra used for the liver disease diagnosis[J].Laser Technology,2005,29(6):579-581(in Chinese).
[11]	LAN X F,LIU Y,GAO Sh M.Analysis of fluorescence spectra of alcohol and its characteristic[J].Laser Technology,2003,27(5):477-479 (in Chinese).
[12]	WANG K,ZHANG X Zh,WANG Zh H,et al.Enhancement of SNR and precision in measurement of time resolved fluorescence spectra[J].Laser Technology,2005,29(2):126-129(in Chinese).
[13]	LI R Q,LIU Y,GE L X,et al.Study on blood cells disintegration by laser included fluorescence spectrometry[J].Acta Photonica Sinica,2006,35(3):398-401(in Chinese).
[14]	RENEE D J,GRAY A C,KARL S B.Excitation-emission matrix fluorescence based determination of carbamate pesticides and polycyclic aromatic hydrocarbons[J].Analytica Chimica Acta,1999,397(1):61-72.
[15]	VILCHEZ J L,EL-KHATTABI R,BLANC R.Photochemical-fluorimetric method for the determination of the insecticide imidaclopried in water samples[J].Analytica Chimica Acta,1998,371(3):247-253.
[16]	WANG Zh D,WANG Y T.Theoretical and experimental study on fluorescence characteristics of common pesticides[J].Chinese Journal of Luminescence,2005,26(1):120-124(in Chinese).
[17]	WANG Zh D,LI D M,WANG Y T.Study on the detection of organic pesticides by fluorescence spectra[J].Spectroscopy and Spectral Analysis,2007,27(11):2321-2324(in Chinese).
[18]	YOSHII K,KAIHARE A,TSUMURA Y,et al.Liquid chromatographic determination of emamectin,milbemectin,ivermectin and abamectin in crops and confirmation by liquid chromatography mass spectrometry[J].Journal of Chromatography,2000,A896(1/2):75-85.
[19]	FERNANDEZ M,PICO Y,MANES J.Rapid screening of organophosphorus pesticides in honey and bees by liquid[J].Chromatographia,2002,56(9/10):577-583.
[20]	LIANG Y,LIU X J,LIU Y.Synthesis of three haptens for the class-specific immunoassay of 0,0-dimethyl organophosphorus pesticides and effect of hapten heterology on immunoassay sensitivity[J].Analytica Chimica Acta,2008,615(2):174-183.
[21]	CALZA P,MASSOLINO C,PELIZZETTI E.Light induced transformations of selected organophosphorous pesticides on titanium dioxide:pathways and by-products evaluation using LC-MS technique[J].Journal of Photochemistry and Photobiology,2008,A199(1):42-49.
[22]	WU Zh Y,LI Zh G,LIU X,et al.Application and advance on detection of organophosphorus pesticide[J].Journal of Beijing Technology and Business University(Natural Science Edition),2004,22(4):15-19(in Chinese).
[23]	CHEN G Zh,XU J G.Florescence spectroscopy[M].Beijing:Science Press,2006:18-36(in Chinese).
[24]	LU W M,CHEN F.Spectrum basis and structural analysis[M].Beijing:Higher Education Press,2005:82-85(in Chinese).
[25]	LIU L Y.Theory explanation of the orientation effect of substitutes on benzene by geminate motion viewpoint of excited electron[J].Journal of Minxi Vocational and Technical College,2006,8(1):119-124(in Chinese).
[26]	YUAN Zh P.Quantity estimate of double atomic molecular energy levels[J].Journal of Gannan Teacner's College,1990,11(3):34-38(in Chinese).
[27]	CHEN X J,ZHU T.Quantum analysis of methanol fluorescent spectra[J].Acta Photonica Sinica,2008,37(7):1433-1435(in Chinese).
[28]	ZENG J Y.Tutorial of quantum mechanics[M].Beijing:Science Press,2004:230-231(in Chinese).

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3205) PDF downloads(226) Cited by()

Proportional views

Fluorescence spectra of methyl parathion and its characteristic

Corresponding author: CHEN Guo-qing, cgq2098@163.com;

1. School of Science, Jiangnan University, Wuxi 214122, China

Received Date: 2008-12-08

Accepted Date: 2009-03-05

Available Online: 2010-03-25

Abstract: In order to study the fluorescence spectra of methyl parathion and its characteristics, the spectral characteristics of standard methyl parathion solution induced by UV-light were experimentally studied with a multifunctional spectrometer system.Based on theoretical analysis by means of GAUSSIAN software and the methods of quantum mechanics,the mechanism of the fluorescence spectra of standard methyl parathion solution and the transition energy of methyl parathion were obtained and the transition energy of the linear structure fluorophore was estimated. The experimental valve was a little different from the theoretical value.The results show that there are four fluorescence peaks generated when the standard methyl parathion solution is excited by UV light at the wavelength of 200nm-320mn. The peak position is located at 435nm, 467nm, 567nm and 650nm respectively. The best excitation wavelength is 200nm and 295nm. The four fluorescence peaks are generated by the combined actions of the methyl parathion and the acetone.In methyl parathion molecule, there are the transition of n electrons in nitryl, the transition of electronic structure formed by the oxygen atom and the benzene ring and the transition of n electrons in P=S group.In acetone molecule, there is the transition of n electrons in C=O group. These results are helpful for testing organophosphorus pesticide's content in the area such as food safety inspection.

HTML

Reference (28)

[1]	YANG Y L,LIU H L.Application study of fluorescent analysis on pesticide residue detection[J].Life Science Instruments,2007,5(9):9-13(in Chinese).
[2]	DENG Y H.To detect trace organic phosphorus pesticide using GC-pusle FPD[J].Administration and Technique of Environmental Monitoring,2003,15(3):24-26(in Chinese).
[3]	SU J F,LIN G Y,CHEN J X,et al.Determination of residual cumyluron in vegerable by solid phase extraction with gas chromatography/mass spectrometry and its fragmentation mechanism study[J].Chinese Journal of Analytical Chemistry,2008,36(4):545-548(in Chinese).
[4]	HE A A,ZHAO J,DONG X H,et al.Determination of residual carbendazim,thiabendazile,imidacloprid and acetamiprid in apple and apple juice concentrate by HPLC[J].Chinese Journal of Analysis Laboratory,2008,27(6):64-67(in Chinese).
[5]	XU X L,LI L,DING G D,et al.Rapid analysis of 13 pesticide residues in rice by large volume injection/gas chromatography-mass spectrometry coupled with solid phase extraction[J].Journal of Instrumental Analysis,2008,27(1):30-33(in Chinese).
[6]	ZHAO Y Y,LIU H F,HAO L,et al.Determination of paraquat in serum by ultraviolet spectrophotometry[J].Journal of Environment and Health,2007,24(5):346-347(in Chinese).
[7]	JACKSON P.Advantages of combined gas chromatograph-mass spectrometry-matrix isolation fourier transform infrared analysis[J].Analytical Proceeding,1993,30(10):135-139.
[8]	QIU Zh,LU J F,HE Y.Rapid detection system development and influence factor analysis for pesticide residues in agricultural products by enzyme inhibition method[J].Transactions of the Chinese Society of Agricultural Engineering,2007,23(9):229-233(in Chinese).
[9]	ROLFD H S.Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction[J].Analytical and Bioanalytical Chemistry,2002,372(2):268-272.
[10]	WU M,DING J H,WANG Ch J.Study on the polarization fluorescence spectra used for the liver disease diagnosis[J].Laser Technology,2005,29(6):579-581(in Chinese).
[11]	LAN X F,LIU Y,GAO Sh M.Analysis of fluorescence spectra of alcohol and its characteristic[J].Laser Technology,2003,27(5):477-479 (in Chinese).
[12]	WANG K,ZHANG X Zh,WANG Zh H,et al.Enhancement of SNR and precision in measurement of time resolved fluorescence spectra[J].Laser Technology,2005,29(2):126-129(in Chinese).
[13]	LI R Q,LIU Y,GE L X,et al.Study on blood cells disintegration by laser included fluorescence spectrometry[J].Acta Photonica Sinica,2006,35(3):398-401(in Chinese).
[14]	RENEE D J,GRAY A C,KARL S B.Excitation-emission matrix fluorescence based determination of carbamate pesticides and polycyclic aromatic hydrocarbons[J].Analytica Chimica Acta,1999,397(1):61-72.
[15]	VILCHEZ J L,EL-KHATTABI R,BLANC R.Photochemical-fluorimetric method for the determination of the insecticide imidaclopried in water samples[J].Analytica Chimica Acta,1998,371(3):247-253.
[16]	WANG Zh D,WANG Y T.Theoretical and experimental study on fluorescence characteristics of common pesticides[J].Chinese Journal of Luminescence,2005,26(1):120-124(in Chinese).
[17]	WANG Zh D,LI D M,WANG Y T.Study on the detection of organic pesticides by fluorescence spectra[J].Spectroscopy and Spectral Analysis,2007,27(11):2321-2324(in Chinese).
[18]	YOSHII K,KAIHARE A,TSUMURA Y,et al.Liquid chromatographic determination of emamectin,milbemectin,ivermectin and abamectin in crops and confirmation by liquid chromatography mass spectrometry[J].Journal of Chromatography,2000,A896(1/2):75-85.
[19]	FERNANDEZ M,PICO Y,MANES J.Rapid screening of organophosphorus pesticides in honey and bees by liquid[J].Chromatographia,2002,56(9/10):577-583.
[20]	LIANG Y,LIU X J,LIU Y.Synthesis of three haptens for the class-specific immunoassay of 0,0-dimethyl organophosphorus pesticides and effect of hapten heterology on immunoassay sensitivity[J].Analytica Chimica Acta,2008,615(2):174-183.
[21]	CALZA P,MASSOLINO C,PELIZZETTI E.Light induced transformations of selected organophosphorous pesticides on titanium dioxide:pathways and by-products evaluation using LC-MS technique[J].Journal of Photochemistry and Photobiology,2008,A199(1):42-49.
[22]	WU Zh Y,LI Zh G,LIU X,et al.Application and advance on detection of organophosphorus pesticide[J].Journal of Beijing Technology and Business University(Natural Science Edition),2004,22(4):15-19(in Chinese).
[23]	CHEN G Zh,XU J G.Florescence spectroscopy[M].Beijing:Science Press,2006:18-36(in Chinese).
[24]	LU W M,CHEN F.Spectrum basis and structural analysis[M].Beijing:Higher Education Press,2005:82-85(in Chinese).
[25]	LIU L Y.Theory explanation of the orientation effect of substitutes on benzene by geminate motion viewpoint of excited electron[J].Journal of Minxi Vocational and Technical College,2006,8(1):119-124(in Chinese).
[26]	YUAN Zh P.Quantity estimate of double atomic molecular energy levels[J].Journal of Gannan Teacner's College,1990,11(3):34-38(in Chinese).
[27]	CHEN X J,ZHU T.Quantum analysis of methanol fluorescent spectra[J].Acta Photonica Sinica,2008,37(7):1433-1435(in Chinese).
[28]	ZENG J Y.Tutorial of quantum mechanics[M].Beijing:Science Press,2004:230-231(in Chinese).

Fluorescence spectra of methyl parathion and its characteristic

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views