Characteristics and mechanism for fluorescence spectroscopy of female urine

LU Jun; GAO Shu-mei; XIONG Jie; YANG You-yi; CHEN Guo-qing

doi:10.3969/j.issn.1001-3806.2010.01.013

In order to study the difference of urine fluorescence spectroscopy from healthy female, excited by 200nm～390nm ultraviolet light,the urine specimens of healthy women aging from 20～55 were measured with SP-2558 multifunctional spectrometer system. According to the multi-peak fitting on the line shape of spectrum,the characteristics and mechanism for urine spectrum were found. The results show that the strong urine fluorescence is caused by three inclusions: nicotinamide ade-nine nucleotide (NAD+) which is the metabolic intermediate of kidney, riboflavin and coproporphyrin. Their corresponding peaks are at 458nm,520nm,612nm and 674nm,and the latter two are generated by coproporphyrin. The difference between samples of youth and elderly is the peak intensity at 458nm, for the elderly women's contends of NAD+ in the urine is higher than that of the young due to the degeneration of kidney metabolic function.

Characteristics and mechanism for fluorescence spectroscopy of female urine

Corresponding author: GAO Shu-mei, gaosm@jianguan.edu.cn;

1. School of Science, Jiangnan University, Wuxi 214122, China;

2. Wuxi Hospital for Matemaland Child Health Care, Wuxi 214122, China

Received Date: 2008-09-22

Accepted Date: 2008-11-04

Available Online: 2010-01-25

Abstract: In order to study the difference of urine fluorescence spectroscopy from healthy female, excited by 200nm～390nm ultraviolet light,the urine specimens of healthy women aging from 20～55 were measured with SP-2558 multifunctional spectrometer system. According to the multi-peak fitting on the line shape of spectrum,the characteristics and mechanism for urine spectrum were found. The results show that the strong urine fluorescence is caused by three inclusions: nicotinamide ade-nine nucleotide (NAD+) which is the metabolic intermediate of kidney, riboflavin and coproporphyrin. Their corresponding peaks are at 458nm,520nm,612nm and 674nm,and the latter two are generated by coproporphyrin. The difference between samples of youth and elderly is the peak intensity at 458nm, for the elderly women's contends of NAD+ in the urine is higher than that of the young due to the degeneration of kidney metabolic function.

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Reference (15)

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Characteristics and mechanism for fluorescence spectroscopy of female urine

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