石英微通道内测量细胞表面电荷的研究

陈德良; 冯彩玲; 秦水介

doi:10.3969/j.issn.1001-3806.2009.06.001

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石英微通道内测量细胞表面电荷的研究

陈德良 , 冯彩玲 , 秦水介

文章导航 > 激光技术 > 2009 > 33(6): 561-564

引用本文:

Citation:

石英微通道内测量细胞表面电荷的研究

1.
贵州大学贵州省光电子技术与应用重点实验室, 贵阳, 550025

作者简介: 陈德良(1980-),男,硕士研究生,主要从事激光技术与应用的学习与研究..

通讯作者: 秦水介, sjqin@mail.gzst.gov.cn

基金项目:
国家自然科学基金资助项目(60666001);贵州省科学技术基金资助项目(黔科合J字[2007]2201)
中图分类号: Q631;Q66

Study on measuring surface-charges of cells in a quartz microchannel

1.
Laboratory for Photoelectric Technology and Application of Guizhou, Guizhou University, Guiyang 550025, China

Corresponding author: QIN Shui-jie, sjqin@mail.gzst.gov.cn

CLC number: Q631;Q66

摘要: 为了具体测量细胞表面电荷量,设计了一种利用光镊、微通道和电泳测量细胞表面电荷的方法,并利用该方法测量得到酵母菌细胞表面电荷大约为10-10℃.结果表明,可以利用该方法测量细胞表面电荷变化.对测量系统进一步开发,可做成测量细胞表面电荷的生物分析芯片.
- 医用光学与生物技术 /
- 细胞表面电荷 /
- 光镊 /
- 石英微通道 /
- 电泳
Abstract: The surface-charges of cells are very important to study the vital characteristic of cells.Common methods only measure the changes of cell electrophoresis mobility (EPM) instead of the quantity of cell surface-charges.In order to measure the surface-charges of cells,a novel method to measure the surface-charges of cells by means of optical tweezers and electrophoresis in a microchannel was designed.With this method,the quantity of the electric charge of yeast cells was measured about 10-10℃.Experimental results prove that this method can be used to measure the changes of cell surface-charges.A lab-onchip (LOC) for measuring the surface-charge of cells might be developed after improving this measuring system.
- medical optics and biotechnology /
- surface-charges of cells /
- optical tweezers /
- quartz microchannels /
- electrophoresis

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