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暗适应ERG波形中有可辨认的给光反应b波和撤光反应d波。b波、d波分别为图 2所示的正波和负波,选用波形尖锐、振幅较大的b波作为眩目效应恢复的评价指标。激光照射后,ERG波形有一过性消失或振幅减低,即表明有眩目效应发生。激光照射后ERG中波形恢复时间变化如图 2所示,不同剂量激光照射后ERG如图 3所示。
持续动态记录兔眼ERG波形图,并测量不同波长不同剂量激光照射后ERG中b波的恢复时间。从图 2中可以看出,照后即刻出现一个比较尖的电信号,随后b波和d波出现一过性消失,随即b波和d波缓慢恢复,b波开始恢复时幅度很小,而后逐渐增高,直至恢复到照前水平,恢复时间即为两个b波波峰对应时间相减所得。激光照射后ERG中b波恢复时间的平均值、最小值、最大值以及标准差如表 1所示。
Table 1. Recovery time of b-wave in ERG after laser irradiation
predicted dose/(mJ·cm-2) average time/s minimum time/s maximum time/s standard deviation 0.020 1.11 0.6 1.60 0.37 0.050 1.59 1.08 2.60 0.45 0.100 2.10 1.56 3.64 0.53 0.200 3.50 2.46 3.72 0.66 0.499 3.97 2.50 5.08 0.71 0.998 4.82 2.76 6.78 0.91 3.494 7.38 4.60 8.30 1.16 5.990 8.77 5.84 10.62 1.12 8.985 9.87 7.00 12.40 1.32 11.980 12.01 8.98 12.70 0.90 13.997 13.55 8.68 12.90 1.30 16.015 14.52 8.69 15.30 1.62 暗适应条件下,蓝激光不同剂量致眩ERG b波恢复时间如图 4所示。兔眼眩目ERG b波平均恢复时间随着激光剂量的增加而增加。低剂量致眩时间不长,但增幅较大,超过0.2mJ/cm2剂量时所致眩目时间呈线性递增,在大剂量时激光致眩时间可达15s左右。
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激光眩目实验后,用眼底照相机观察视网膜损伤表现,未见激光损伤斑。由此可见,上述激光致眩所用照射剂量不会引起眼组织的器质性损伤。致眩实验后兔眼视网膜眼底照片如图 5所示。其中图 5a为照前眼底照片,图 5b为照后即刻眼底照片,图 5c为照后24h眼底照片。
456nm半导体蓝激光眩目效应研究
Study on the dazzle effect of 456nm blue laser
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摘要: 为了探讨蓝激光眩目效应量效关系,利用视网膜电流图研究456nm半导体蓝激光照射兔眼的眩目效应,通过视网膜电流图b波振幅恢复时间评价蓝激光致眩效果,绘制剂量-恢复时间曲线,并通过眼底照相机观察激光辐照后的视网膜损伤情况。结果表明,456nm蓝激光眩目效果明显,视网膜电流图b波振幅的恢复时间随蓝激光辐照剂量的增加而延长,恢复时间与剂量间呈良好的线性关系,辐照后即刻和辐照24h后观察眼底未见损伤。本研究将为蓝激光的眩目应用提供了参考。Abstract: In order to explore the dose effect relationship of blue laser dazzle effect, electroretinogram was used to evaluate the dazzle effect of a 456nm semiconductor blue laser on rabbit eyes. The dazzle effect of blue laser and its characteristics were evaluated by the recovery time of electroretinogram b-wave amplitude, the dazzling recovery time curves corresponding to different doses were drawn, and the damage of retina after laser irradiation was observed with fundus camera. The results show that the recovery time of b-wave amplitude of electroretinogram (ERG) is prolonged with the increase of laser irradiation dose, and there is a good linear relationship between the recovery time and the dose. No damage is found in the fundus immediately after irradiation and 24h later. This study will provide reference value for the application of blue laser dazzle.
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Key words:
- medical optics /
- dazzle effect /
- electroretinogram /
- blue laser /
- semiconductor laser
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Table 1. Recovery time of b-wave in ERG after laser irradiation
predicted dose/(mJ·cm-2) average time/s minimum time/s maximum time/s standard deviation 0.020 1.11 0.6 1.60 0.37 0.050 1.59 1.08 2.60 0.45 0.100 2.10 1.56 3.64 0.53 0.200 3.50 2.46 3.72 0.66 0.499 3.97 2.50 5.08 0.71 0.998 4.82 2.76 6.78 0.91 3.494 7.38 4.60 8.30 1.16 5.990 8.77 5.84 10.62 1.12 8.985 9.87 7.00 12.40 1.32 11.980 12.01 8.98 12.70 0.90 13.997 13.55 8.68 12.90 1.30 16.015 14.52 8.69 15.30 1.62 -
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